Synthesis and polymerase incorporation of 5'-amino-2',5'-dideoxy-5'-N-triphosphate nucleotides

Inhibition of Ribonucleic Acid Polymerase by 5-Hydroxyuridine 5’-Triphosphate*

5-Hydroxyuridine triphosphate was synthesized and its effectiveness as substrate for deoxyribonucleic acid-dependent ribonucleic acid polymerase was determined. The analogue was incorporated into RNA to a very low extent as compared to naturally occurring nucleoside triphosphates, and it replaced uridine triphosphate to a much greater extent than any of the other nucleoside triphosphates. The a...

Inhibition of ribonucleic acid polymerase by 5-hydroxyuridine 5'-triphosphate.

5-Hydroxyuridine triphosphate was synthesized and its effectiveness as substrate for deoxyribonucleic acid-dependent ribonucleic acid polymerase was determined. The analogue was incorporated into RNA to a very low extent as compared to naturally occurring nucleoside triphosphates, and it replaced uridine triphosphate to a much greater extent than any of the other nucleoside triphosphates. The a...

Role of guanine nucleotides in protein synthesis. Elongation factor G and guanosine 5'-triphosphate,3'-diphosphate.

The possible role of guanosine 5'-triphosphate,3'-diphosphate (pppGpp) in protein synthesis by Escherichia coli ribosomes and protein factors was examined. Although pppGpp could effectively substitute for GTP in reactions catalyzed by initiation factor 2 (ribosomal binding of fMet-tRNA and formation of N-formylmethionylpuromycin) and elongation factor T (ribosomal binding of Phe-tRNA and format...

The incorporation of sangivamycin 5'-triphosphate into polyribonucleotide by ribonucleic acid polymerase from Micrococcus lysodeikticus.

Sangivamycin is incorporated into RNA in the reaction catalyzed by the RNA polymerase from Micrococcus lysodeikticus with the use of calf thymus DNA or the copolymer of deoxyadenosine and deoxythymidine (poly d(A-T)). The incorporation of sangivamycin 5’4riphosphate into RNA is dependent on primer, time, protein concentration, and Mn++ or Mg+f. When calf thymus DNA is primer, GTP, CTP, and UTP ...

Aminoglycoside antibiotics: synthesis of 5''-amino-5''-deoxyneomycin and 5''-amino-5''-deoxyparomomycin.